A practical guide to peptide identity, HPLC purity, net peptide content and the scope of batch-specific analytical reports.
A useful product record connects identity, test method and batch information.
Mass spectrometry can support an expected molecular identity. Chromatography separates components under defined conditions. The method, detector and integration approach influence a reported chromatographic purity value.
Water and counterions can contribute to the gross amount of a preparation. A chromatographic percentage does not directly quantify the peptide share of the weighed material. Use the appropriate reported attribute for the calculation or assay.
Do not infer sterility, absence of endotoxin or suitability for a particular experiment from identity or HPLC results alone. Look for the tests, methods and acceptance criteria that address your question.
The useful document is the one that corresponds to the actual sample. Retain its identifier and report alongside receiving and handling records.
Further reading: GenScript: selecting peptide purity.
New catalogue entries, documentation updates and practical reading.